Στατιστική ανάλυση πειραματικών δεδομένων από αυτοματοποιημένη και υπερευαίσθητη ποσοτικοποίηση θραύσης DNA σε ευκαρυωτικά κύτταρα για την βιολογική εκτίμηση της επικινδυνότητας της ιοντίζουσας ακτινοβολίας χαμηλής δόσης
Statistical analysis of experimental data from an automated and hypersensitive quantification of DNA damage in eukaryotic cells for the biological assesment of the low dose ionizing radiation risk
KeywordsΙοντίζουσα ακτινοβολία ; Ευκαρυωτικά κύτταρα ; Βιολογικοί δείκτες δοσιμετρίας ; Απεικονιστική ιατρική εξέταση ; Επίδραση ακτινοβολίας ; Κίνδυνος ακτινοβολίας ; Καρκινογένεση ; Πανοραμική εξέταση ; Στατιστική ανάλυση ; Υψηλή τεχνολογία
The health of humans as well as the proper function of the eukaryotic cellsall of the organisms are affected by the cumulative radiation exposure and by the instantaneous excessive exposure radiation. The ionizing radiation, according to the global bibliography may cause DNA mutation and DNA breaks, which is possible to result in a possible condition of a carcinogenicity. This master’s dissertation focuses on the humans oral cells dysfunction, owing to use of the ionizing radiation to them. This radiation is used to medical scanning methods, such us panoramic dental scanning. Considering that there is not defining official safety limits of the ionizing radiation levels, it takes place an experiment, a trial in which three groups of oral epithelial cells are ionized by three different values of radiation. The radiation defined in zero, low and the high dose of the ionizing radiation. Then, it came up experimental datas, of which the number of the γ-H2AX histones in two times after the cell’s radiation. The γ-H2AX histones were fluorinated and they detected by the immunofluorescence method. Molecular biology is helpful to understand that after the double strand DNA breaks, in the cell function normally are produced the γ-H2AX histones to restore the DNA breaks. This function is useful for the researchers to estimate the double DNA breaks. Double stranded DNA breaks are the most importantfor the operational main function of a cell, a possible failure to repair them, it can lead to possible carcinogenesis. The experimental measurements collected with the aid of high technology method. Then, it accomplished a statistical processing of the biodata with the help of the statistical package R-Studio. The trial’s data are used for the effort to extrapolate and make useful observations to the correlation and the association between the ionizing radiation, the critical time of the minutes after the radiation moment and the risk of the biological hazard of the radiation. The ultimate purpose of this study is to evaluate the radiation values which are used in the experiment and to study the risk of exposure to the ionizing radiation, which it will based on the formation of the reliable dosimetry biomarkers.