Στατιστική ανάλυση πειραματικών δεδομένων από αυτοματοποιημένη και υπερευαίσθητη ποσοτικοποίηση θραύσης DNA σε ευκαρυωτικά κύτταρα για την βιολογική εκτίμηση της επικινδυνότητας της ιοντίζουσας ακτινοβολίας χαμηλής δόσης
Statistical analysis of experimental data from an automated and hypersensitive quantification of DNA damage in eukaryotic cells for the biological assesment of the low dose ionizing radiation risk
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Keywords
Ιοντίζουσα ακτινοβολία ; Ευκαρυωτικά κύτταρα ; Βιολογικοί δείκτες δοσιμετρίας ; Απεικονιστική ιατρική εξέταση ; Επίδραση ακτινοβολίας ; Κίνδυνος ακτινοβολίας ; Καρκινογένεση ; Πανοραμική εξέταση ; Στατιστική ανάλυση ; Υψηλή τεχνολογίαAbstract
The health of humans as well as the proper function of the eukaryotic cellsall of the
organisms are affected by the cumulative radiation exposure and by the instantaneous
excessive exposure radiation. The ionizing radiation, according to the global
bibliography may cause DNA mutation and DNA breaks, which is possible to result in a
possible condition of a carcinogenicity. This master’s dissertation focuses on the
humans oral cells dysfunction, owing to use of the ionizing radiation to them. This
radiation is used to medical scanning methods, such us panoramic dental scanning.
Considering that there is not defining official safety limits of the ionizing radiation
levels, it takes place an experiment, a trial in which three groups of oral epithelial cells
are ionized by three different values of radiation. The radiation defined in zero, low and
the high dose of the ionizing radiation. Then, it came up experimental datas, of which
the number of the γ-H2AX histones in two times after the cell’s radiation. The γ-H2AX
histones were fluorinated and they detected by the immunofluorescence method.
Molecular biology is helpful to understand that after the double strand DNA breaks, in
the cell function normally are produced the γ-H2AX histones to restore the DNA
breaks. This function is useful for the researchers to estimate the double DNA breaks.
Double stranded DNA breaks are the most importantfor the operational main function
of a cell, a possible failure to repair them, it can lead to possible carcinogenesis. The
experimental measurements collected with the aid of high technology method. Then, it
accomplished a statistical processing of the biodata with the help of the statistical
package R-Studio. The trial’s data are used for the effort to extrapolate and make useful
observations to the correlation and the association between the ionizing radiation, the
critical time of the minutes after the radiation moment and the risk of the biological hazard of the radiation. The ultimate purpose of this study is to evaluate the radiation
values which are used in the experiment and to study the risk of exposure to the ionizing
radiation, which it will based on the formation of the reliable dosimetry biomarkers.